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Regeneration Protocol for Ni-NTA/IDA Agarose by Chely López is a document available to read on EtoBox.

What is Regeneration Protocol for Ni-NTA/IDA Agarose about?

This document outlines the washing and regeneration procedures for Ni-NTA and Ni-IDA agarose resins, recommending washing after each use and regeneration after five runs. It provides detailed instructions for preparing various buffers and solutions, as well as specific steps for handling resins exposed to reducing agents like DTT. The protocol is applicable for different metal-loaded resins and includes contact information for further assistance.

Author

Chely López

Language

EN