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Can I read Weightlessness acts on human breast cancer cell line MCF-7 on EtoBox?
Weightlessness acts on human breast cancer cell line MCF-7 by J. Vassy; S. Portet; M. Beil; G. Millot; F. Fauvel-Lafève; G. Gasset; D. Schoevaert is a Physics and Astronomy article available to read on EtoBox.
What is Weightlessness acts on human breast cancer cell line MCF-7 about?
Because cells are sensitive to mechanical forces, weightlessness might act on stress-dependent cell changes. Human breast cancer cells MCF-7, flown in space in a Photon capsule, were fixed after 1.5, 22 and 48 h in orbit. Cells subjected to weightlessness were compared to lg in-flight and ground controls. Post-flight, fluorescent labeling was performed to visualize cell proliferation (Ki-67) three cytoskeleton components and chromatin structure. Confocal microscopy and image analysis were used to quantify cycling cells and mitosis, modifications of the cytokeratin network and chromatin structure. Several main phenomena were observed in weightlessness: -The perinuclear cytokeratin network and chromatin structure were looser. -More cells were cycling and mitosis was prolonged. Finally, cell proliferation was reduced as a consequence of a cell-cycle blockade. -Microtubules were altered in many cells. The results reported in the first point are in agreement with basic predictions of cellular tensegrity. The prolongation of mitosis can be explained by an alteration of microtubules. We discuss here the different mechanisms involved in weightlessness alteration of microtubules: i) alterat
Who reads Weightlessness acts on human breast cancer cell line MCF-7?
It is typically read by researchers, students, and practitioners in Physics and Astronomy.
- Author
- J. Vassy; S. Portet; M. Beil; G. Millot; F. Fauvel-Lafève; G. Gasset; D. Schoevaert
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0273-1177)
- Published
- 2003
- Language
- EN
- Field
- Physics and Astronomy (Physical Sciences)