Can I read Immunoelectron Microscopy: Methods and Protocols (Methods in Molecular Biology, 657) on EtoBox?

Immunoelectron Microscopy: Methods and Protocols (Methods in Molecular Biology, 657) by David M. Rancour, Steven K. Backues, Sebastian Y. Bednarek (auth.), Steven D. Schwartzbach, Tetsuaki Osafune (eds.) is a nonfiction available to read on EtoBox.

What is Immunoelectron Microscopy: Methods and Protocols (Methods in Molecular Biology, 657) about?

Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Mol

Who reads Immunoelectron Microscopy: Methods and Protocols (Methods in Molecular Biology, 657)?

Author

David M. Rancour, Steven K. Backues, Sebastian Y. Bednarek (auth.), Steven D. Schwartzbach, Tetsuaki Osafune (eds.)

Publisher

Humana Press : Imprint : Humana Press

Published

2010

Language

EN

ISBN

9781607617839

Category

nonfiction

Subjects

Science, Technology, Chemistry