Can I read Mapping Sites Responsible for Interactions of Agrin with Neurons on EtoBox?

Mapping Sites Responsible for Interactions of Agrin with Neurons by Robert W. Burgess; Dion K. Dickman; Lorna Nunez; David J. Glass; Joshua R. Sanes is a Neuroscience article available to read on EtoBox.

What is Mapping Sites Responsible for Interactions of Agrin with Neurons about?

## Abstract The multidomain proteoglycan agrin is a critical organizer of postsynaptic differentiation at the skeletal neuromuscular junction. Agrin is also abundant in the brain, but its roles there are unknown. As a step toward understanding these roles, we mapped sites responsible for interactions of neurons with agrin. First, we used a series of recombinant agrin fragments to show that at least four sites on agrin interact with chick ciliary neurons. Use of blocking antibodies and peptides indicated that neurons adhere to a site in the second of three G domains by means of αVβ1 integrin, and to a site in the last of four epidermal growth factor (EGF) repeats via a distinct β1 integrin. A third, integrin‐independent adhesion site is near to but distinct from the site that induces postsynaptic differentiation in muscles. These domains are insufficient, however, to account for neurite outgrowth‐inhibiting properties of full‐length agrin, which are mediated by the N‐terminal half of the molecule. We then used a second set of agrin mutants to demonstrate and map a transmembrane domain in the amino‐terminus of the SN‐isoform of agrin. The extracellular matrix‐bound form of agrin, cal

Who reads Mapping Sites Responsible for Interactions of Agrin with Neurons?

Author

Robert W. Burgess; Dion K. Dickman; Lorna Nunez; David J. Glass; Joshua R. Sanes

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

2002

Language

EN

Field

Neuroscience (Life Sciences)