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Ascorbate-dependent and ascorbate-independent Mn porphyrin cytotoxicity: anticancer activity of Mn porphyrin-based SOD mimics through ascorbate-dependent and -independent routes by Bader Hasan; Artak Tovmasyan; Ines Batinic-Haberle; Ludmil Benov is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## ABSTRACT ## Objective The aim of this study was to investigate how modifications at the periphery of the porphyrin ring affect the anticancer activity of Mn porphyrins (MnPs)-based SOD mimics. ## Methods Six compounds: MnTE-2-PyP with a short ethyl chain on the pyridyl ring; MnTnHexOE-2-PyP and MnTnOct-2-PyP with linear 8-atom alkyl chains, but the former with an oxygen atom within the alkyl chain; MnTE-2-PyPhP and MnTPhE-2-PyP with pyridyl and phenyl substituents, were investigated. Cytotoxicity was studied using pII and MDA-MB-231 cancer cell lines. Viability was assessed by the MTT (3-[4,5-dimethylthiazol-2-yl)]-2,5-diphenyltetrazolium bromide) assay and cell proliferation was determined by the sulforhodamine B assay. ## Results Cellular uptake was increased with the increase of the lipophilicity of the compounds, whereas reduction potential (__E__~1⁄2~) of the Mn(III)/Mn(II) redox couple shifted away from the optimal value for efficient redox cycling with ascorbate, necessary for ROS production. Amphiphilic MnPs, however, exerted anticancer activity by a mechanism not involving ROS. ## Conclusion Two different processes account for MnPs cytotoxicity. MnPs with appropriate __

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Author

Bader Hasan; Artak Tovmasyan; Ines Batinic-Haberle; Ludmil Benov

Publisher

Informa UK Limited

Published

2021

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)