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Glutamate receptor, ionotropic, kainate 2 silencing by DNA hypermethylation possesses tumor suppressor function in gastric cancer by Chi-Sheng Wu; Yen-Jung Lu; Hsin-Pai Li; Chuen Hsueh; Chang-Yi Lu; Yu-Wei Leu; Hao-Ping Liu; Kwang-Huei Lin; Tim Hui-Ming Huang; Yu-Sun Chang is a Medicine article available to read on EtoBox.
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## Abstract Aberrant DNA methylation is considered a major mechanism for silencing tumor suppressor genes in gastric cancer. We used CpG microarray and differential methylation hybridization strategies to identify potential tumor suppressor genes and recovered glutamate receptor, ionotropic, kainate 2 (GRIK2) as a novel epigenetic target in gastric cancer. Additional experiments showed that the promoter region of __GRIK2__ was hypermethylated in 3 of the 4 tested gastric cancer cell lines, and its expression was restored by treatment of cells with the DNA methylation inhibitor, 5′‐aza‐dC. In clinical samples, the __GRIK2__ promoter was differentially hypermethylated in tumor tissues compared with adjacent normal tissues (__p__ < 0.001), and this methylation was inversely correlated with the expression level of __GRIK2__ mRNA (__r__ = −0.44). Functional studies further showed that GRIK2‐expressing gastric cancer cell lines showed decreased colony formation and cell migration. Taken together, these results suggest that GRIK2 may play a tumor‐suppressor role in gastric cancer. Future studies are warranted to examine whether DNA hypermethylation of the __GRIK2__ promoter can be used as
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- Author
- Chi-Sheng Wu; Yen-Jung Lu; Hsin-Pai Li; Chuen Hsueh; Chang-Yi Lu; Yu-Wei Leu; Hao-Ping Liu; Kwang-Huei Lin; Tim Hui-Ming Huang; Yu-Sun Chang
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2010
- Language
- EN
- Field
- Medicine (Health Sciences)