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Identification and Localization of Integrin Subunits in Oocytes and Eggs of the Mouse by Janice P. Evans; Richard M. Schultz; Gregory S. Kopf is a Medicine article available to read on EtoBox.

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## Abstract Results of a recent study have implicated egg integrins in sperm binding to the egg plasma membrane (Blobel et al., 1991: Nature 356:248–252). In this report, immunoprecipitation was used to identify, and confocal immunofluorescence microscopy was used to localize, several different integrin subunits in mouse eggs. Antibodies to α~2~, α~5~, α~v~, and β~1~ subunits, as well as antibodies to the fibronectin receptor (FNR; α~5~β~1~ and/or α~3~β~1~) and vitronectin receptor (VNR; α~v~β~3~ and/or α~v~β~5~), detect polypeptides of the appropriate molecular weights following immunoprecipitation. β~1~ is localized preferentially to either the microvillar or amicrovillar membrane/cortical regions of eggs, and these asymmetric localizations depend on the antibody used. Proteins recognized by anti‐FNR antibodies are localized preferentially to the amicrovillar membrane/cortical region. Germinal vesicle‐intact oocytes display a symmetric plasma membrane distribution using β and FNR antibodies, and the asymmetric distribution develops as a consequence of oocyte maturation and is clearly observed by metaphase I. In contrast to the membrane localization of these integrin subunits, α~2

Who reads Identification and Localization of Integrin Subunits in Oocytes and Eggs of the Mouse?

Author

Janice P. Evans; Richard M. Schultz; Gregory S. Kopf

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1040-452X)

Published

1995

Language

EN

Field

Medicine (Life Sciences)