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QTY code-designed water-soluble Fc-fusion cytokine receptors bind to their respective ligands by Hao, Shilei; Jin, David; Zhang, Shuguang; Qing, Rui is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Abstract Cytokine release syndrome (CRS), or ‘cytokine storm’, is the leading side effect during chimeric antigen receptor (CAR)-T therapy that is potentially life-threatening. It also plays a critical role in viral infections such as Coronavirus Disease 2019 (COVID-19). Therefore, efficient removal of excessive cytokines is essential for treatment. We previously reported a novel protein modification tool called the QTY code, through which hydrophobic amino acids Leu, Ile, Val and Phe are replaced by Gln (Q), Thr (T) and Tyr (Y). Thus, the functional detergent-free equivalents of membrane proteins can be designed. Here, we report the application of the QTY code on six variants of cytokine receptors, including interleukin receptors IL4Rα and IL10Rα, chemokine receptors CCR9 and CXCR2, as well as interferon receptors IFNγR1 and IFNλR1. QTY-variant cytokine receptors exhibit physiological properties similar to those of native receptors without the presence of hydrophobic segments. The receptors were fused to the Fc region of immunoglobulin G (IgG) protein to form an antibody-like structure. These QTY code-designed Fc-fusion receptors were expressed in Escherichia coli and purified. Th

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Author

Hao, Shilei; Jin, David; Zhang, Shuguang; Qing, Rui

Publisher

Cambridge University Press (CUP) (ISSN 2633-2892)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)