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Single GABAergic synaptic terminals from rat midbrain exhibit functional P2X and dinucleotide receptors, able to induce GABA secretion by Rosa Gómez-Villafuertes; Javier Gualix; M. Teresa Miras-Portugal is a Neuroscience article available to read on EtoBox.

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GABAergic terminals from rat midbrain characterized by immunolocalization of glutamic acid decarboxylase and/or the vesicular inhibitory amino acid transporter respond to ATP or P^1^,P^5^‐di(adenosine‐5′) pentaphosphate (Ap~5~A) with an increase in the intrasynaptosomal calcium concentration measured by a microfluorimetric technique in single synaptic terminals. The ATP response is mediated through the activation of P2X receptors with an abundant presence of P2X~3~ subunits. Ap~5~A, however, exerts its effects by acting through a different receptor termed the dinucleotide receptor. Both receptors, once activated in the presence of extrasynaptosomal calcium, induce a concentration‐dependent GABA release from synaptosomal populations with EC~50~ values of 16 and 20 μm for ATP and Ap~5~A, respectively. Specific inhibition of GABA release is obtained with pyridoxal phosphate‐6‐azophenyl‐2′,4′‐disulphonic acid (80 μm) on the ATP effect and with P^1^,P^5^‐di(inosine‐5′) pentaphosphate (100 nm) on the dinucleotide receptor.

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Author

Rosa Gómez-Villafuertes; Javier Gualix; M. Teresa Miras-Portugal

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

2001

Language

EN

Field

Neuroscience (Life Sciences)