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A Selective Extraction of Growth Hormone from Bovine Pituitary Gland and Its Further Purification and Crystallization by Vitaly L. Spitsberg is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is A Selective Extraction of Growth Hormone from Bovine Pituitary Gland and Its Further Purification and Crystallization about?
A highly efficient method for the isolation of bovine growth hormone (GH) is described. The method is based on selective extraction of GH from 15,000 g subcellular sediment of anterior pituitary gland with 130-150 mM NH4HCO3, 1 mM EGTA, pH 7.2-7.4, at 2-6 degrees C for 60 min, purification of the extracted GH by ammonium sulfate fractionation, one-step ion-exchange column chromatography on DEAE-cellulose (or CM-cellulose), and gel filtration on Sephadex G-75. This 2-3 day procedure provides a highly pure hormone in high yield (up to 70-80 mg per 35-40 g of the whole pituitary gland), which can be crystallized by the batch method at a low ionic strength and isoelectric pH.
Who reads A Selective Extraction of Growth Hormone from Bovine Pituitary Gland and Its Further Purification and Crystallization?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Vitaly L. Spitsberg
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 1987
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)