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Canonical transient receptor potential channel 4 (TRPC4) co‐localizes with the scaffolding protein ZO‐1 in human fetal astrocytes in culture by Xianyuan Song; Yongmei Zhao; Leontine Narcisse; Heather Duffy; Yvonne Kress; Sunhee Lee; Celia F. Brosnan is a Neuroscience article available to read on EtoBox.
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## Abstract Members of the mammalian transient receptor potential (TRP) family form cation‐permeable channels at the plasma membrane implicated in capacitative calcium influx after activation by either second‐messenger‐mediated pathways or store depletion, or both. This study shows that with the use of RT‐PCR, Western blotting, and immunohistochemistry, resting astrocytes express TRPC4 at the cell membrane, particularly at sites of cell‐to‐cell contact. By confocal imaging and immunoelectron microscopy, we detected co‐localization of TRPC4 with the scaffolding protein zonula occludens 1 (ZO‐1), and demonstrated that immunoprecipitation with antibodies to ZO‐1 brought down TRPC4, and vice‐versa. It has been proposed that the targeting of TRPC4 to the cell membrane is dependent on the interaction of the C‐terminal TRL motif with PDZ domains. Using transfection of astrocytes with myc‐tagged TRPC4 or TRL‐motif truncated TRPC4 (δTRL), we found that δTRL localized predominantly to a juxtanuclear compartment, whereas the wild‐type protein showed cell surface distribution. Deletion of the TRL motif also reduced plasma membrane expression as assessed by cell surface biotinylation experiment
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- Author
- Xianyuan Song; Yongmei Zhao; Leontine Narcisse; Heather Duffy; Yvonne Kress; Sunhee Lee; Celia F. Brosnan
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)
- Published
- 2004
- Language
- EN
- Field
- Neuroscience (Life Sciences)