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Detection of widespread hepatocyte infection in chronic hepatitis C by Vincent Agnello; György Ábel; Glenn B. Knight; Elizabeth Muchmore is a Medicine article available to read on EtoBox.

What is Detection of widespread hepatocyte infection in chronic hepatitis C about?

The controversial question of the extent of hepatocyte infection in chronic hepatitis C was re-examined in both chimpanzees and humans using a newly modified in situ hybridization (ISH) method for detecting hepatitis C virus (HCV) RNA. The specificity of the methodology for distinguishing positive- and negative-strand synthetic HCV RNA was at least six magnitudes greater than the reverse-transcription polymerase chain reaction (RT-PCR) assay for HCV. The sensitivity of the methodology as determined by cell culture assay was 14 +/- 2 genomic equivalents (gE) of HCV positive strand per cell, which was three magnitudes less sensitive than RT-PCR quantitation of HCV. In contrast to previous studies in both humans and chimpanzees with chronic hepatitis C, a high percentage of hepatocytes positive for both positive- and negative-strand HCV RNA was found in most specimens studied. In humans, the extent of hepatocyte infection varied with histological activity index (HAI). In the two chimpanzees studied, the liver biopsies showed minimal histological disease activity, but high percentages of hepatocytes were HCV-positive by ISH that correlated with hepatocyte ultrastructural changes associ

Who reads Detection of widespread hepatocyte infection in chronic hepatitis C?

Author

Vincent Agnello; György Ábel; Glenn B. Knight; Elizabeth Muchmore

Publisher

Ovid Technologies (Wolters Kluwer Health)

Published

1998

Language

EN

Field

Medicine (Health Sciences)