Can I read One Single Standard Substance for the Determination of Multiple Anthraquinone Derivatives in Rhubarb Using High-performance Liquid Chromatography-diode Array Detection on EtoBox?

One Single Standard Substance for the Determination of Multiple Anthraquinone Derivatives in Rhubarb Using High-performance Liquid Chromatography-diode Array Detection by Xiao-Yan Gao; Yong Jiang; Jian-qiu Lu; Peng-Fei Tu is a Chemistry article available to read on EtoBox.

What is One Single Standard Substance for the Determination of Multiple Anthraquinone Derivatives in Rhubarb Using High-performance Liquid Chromatography-diode Array Detection about?

Due to the complexity of the chemical constitution of traditional Chinese medicines (TCMs), now there is a trend to establish methods, using multi-components analysis, for the effective quality control of TCMs. However, the limited availability of multiple reference substances hinders the wide popularization for routine quality control of TCMs. Using an easily available single component contained in the analyte as an internal standard to determine multiple analogues should be a practical option. In this study, we selected rhubarb rhizome as an example, and used emodin, the easily available active component, as the external standard to determine directly the content of emodin in rhubarb, and the same component as the internal standard to simultaneously determine the other six anthraquinones in rhubarb. Compared with the results obtained using the external standard method, this alternative method was found to have no statistically significant differences in our laboratory as verified by the F-test (p=95%, n=6). However, due to weak robustness caused by the fluctuation of relative response factors in different laboratories, such a method can only serve as a practical measure to solve

Who reads One Single Standard Substance for the Determination of Multiple Anthraquinone Derivatives in Rhubarb Using High-performance Liquid Chromatography-diode Array Detection?

Author

Xiao-Yan Gao; Yong Jiang; Jian-qiu Lu; Peng-Fei Tu

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)

Published

2009

Language

EN

Field

Chemistry (Physical Sciences)