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Can I read Development of a Quantitative High-performance Liquid Chromatography–photodiode Array Detection Measurement System for Phenolic Acids on EtoBox?
Development of a Quantitative High-performance Liquid Chromatography–photodiode Array Detection Measurement System for Phenolic Acids by Rebecca J Robbins; Scott R Bean is a Chemistry article available to read on EtoBox.
What is Development of a Quantitative High-performance Liquid Chromatography–photodiode Array Detection Measurement System for Phenolic Acids about?
A quantitative high-performance liquid chromatography-photodiode array detection method separating 16 phenolic acids was achieved. Six columns and several mobile phases were investigated. Resolution was achieved with a high-purity silica Phenomenex Luna C 18 column (150 mm × 4.6 mm, 5 m) and a binary gradient consisting of CH 3 OH-water (with 0.1% formic acid) and flow rate set at 0.7 ml/min. Acids were detected and quantitation performed at wavelength representing the lowest energy λ max for individual acids. Extraction procedure from wine was optimized and yields ranged from 79 to 87% based on internal standard recovery. To confirm our quantitative results, identical samples were analyzed both in-house and by a collaborating laboratory. Correlation of two data sets generated linear regression equations that approached unity (0.93-0.98) and R 2 values ranging from 0.990 to 0.999.
Who reads Development of a Quantitative High-performance Liquid Chromatography–photodiode Array Detection Measurement System for Phenolic Acids?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Rebecca J Robbins; Scott R Bean
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 2004
- Language
- EN
- Field
- Chemistry (Physical Sciences)