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Homeostatic, repertoire and transcriptional relationships between colon T regulatory cell subsets by Deepshika Ramanan; Kaitavjeet Chowdhary; Serge M. Candéias; Martina Sassone-Corsi; Adelaide Gelineau; Diane Mathis; Christophe Benoist is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Homeostatic, repertoire and transcriptional relationships between colon T regulatory cell subsets about?

Foxp3 ^+^ regulatory T cells (Tregs) in the colon are key to promoting peaceful coexistence with symbiotic microbes. Differentiated in either thymic or peripheral locations, and modulated by microbes and other cellular influencers, colonic Treg subsets have been identified through key transcription factors (TFs; Helios, Rorγ, Gata3, and cMaf), but their interrelationships are unclear. Applying a multimodal array of immunologic, genomic, and microbiological assays, we find more overlap than expected between populations. The key TFs (Rorγ, Helios, Gata3, and cMaf) play different roles, some essential for subset identity, others driving functional gene signatures. Functional divergence was clearest under challenge. Single-cell genomics revealed a spectrum of phenotypes between the Helios+ and Rorγ+ poles, different Treg-inducing bacteria inducing the same Treg phenotypes to varying degrees, not distinct populations. TCR repertoires in monocolonized mice revealed that Helios+ and Rorγ+ Tregs are related and cannot be uniquely equated to tTreg and pTreg. Comparison of spleen and colon repertoires revealed that 2 to 5% of clonotypes are shared between the locations. We propose that rathe

Who reads Homeostatic, repertoire and transcriptional relationships between colon T regulatory cell subsets?

Author

Deepshika Ramanan; Kaitavjeet Chowdhary; Serge M. Candéias; Martina Sassone-Corsi; Adelaide Gelineau; Diane Mathis; Christophe Benoist

Publisher

Proceedings of the National Academy of Sciences

Published

2023

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)