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FGF-1-induced matrix metalloproteinase-9 expression in breast cancer cells is mediated by increased activities of NF-κB and activating protein-1 by Gina Lungu; Lina Covaleda; Odete Mendes; Heidi Martini-Stoica; George Stoica is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract Matrix metalloproteinase‐9 (MMP‐9) plays a critical role in tumor invasion and metastasis. Here, we investigate the effect of fibroblast growth factor‐1 (FGF‐1) on the expression of MMP‐9 in ENU1564, an ethyl‐__N__‐nitrosourea‐induced rat mammary adenocarcinoma cell line. We observed that FGF‐1 induces a dose‐dependent increase in MMP‐9 mRNA, protein, and activity in ENU1564 cells. To gain insight into the molecular mechanism of MMP‐9 regulation by FGF‐1, we investigated the role of components of PI3K‐Akt and MEK1/2‐ERK signaling pathways in our system since NF‐κB and AP‐1 transcription factor binding sites have been characterized in the upstream region of the MMP‐9 gene. We demonstrated that FGF‐1 increases Akt phosphorylation, triggers nuclear translocation of NF‐κBp65, and enhances degradation of cytoplasmic IκBα. Pretreatment of cells with LY294002, a PI3K inhibitor, significantly inhibited MMP‐9 protein expression in FGF‐1‐treated cells. Conversely, our data show that FGF‐1 increases ERK phosphorylation in ENU1564 cells, increases c‐__jun__ and c‐fos mRNA expression in a time‐dependent manner, and triggers nuclear translocation of c‐__jun__. Pretreatment of cells w

Who reads FGF-1-induced matrix metalloproteinase-9 expression in breast cancer cells is mediated by increased activities of NF-κB and activating protein-1?

Author

Gina Lungu; Lina Covaleda; Odete Mendes; Heidi Martini-Stoica; George Stoica

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0899-1987)

Published

2007

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)