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Long term presence of a single predominant tyrosinase‐specific T‐cell clone associated with disease control in a patient with metastatic melanoma by Sebastian Ochsenreither; Alberto Fusi; Antonia Busse; Anne Letsch; Doreen Haase; Eckhard Thiel; Carmen Scheibenbogen; Ulrich Keilholz is a Medicine article available to read on EtoBox.
What is Long term presence of a single predominant tyrosinase‐specific T‐cell clone associated with disease control in a patient with metastatic melanoma about?
## Abstract In an earlier study, we described a patient who developed an anti‐tyrosinase T‐cell response leading to long‐term tumor control. Here we analyzed this response with regard to T‐cell receptor (TCR) __Vβ__ family usage and clonality in order to further elucidate the nature of the T cell response in this patient. For identification of expanded specific cytotoxic T‐cell (CTL) clones, tetramer enrichment of tyrosinase reactive T‐cells was followed by comparative quantitative reverse transcribed PCR (qRT PCR) quantification of all TCR __Vβ__‐families and sequencing of family __Vβ__4 elevated in the enriched fraction. The predominant specific clone was quantified by clonotypic qRT PCR in multiple samples from blood, bone marrow, and tumor tissue. FACS analyses with staining of TYR.A2 and TCR __Vβ__4 were performed. Epitope specific enrichment revealed an isolated increase of __Vβ__−family 4. FACS analysis showed a shift of specific CTLs to __Vβ__‐family 4 during tumor regression with a maximum of 80% of all TYR.A2 specific cells belonging to this family. Sequencing revealed a single predominant clone against polyclonal background coding for identical CDR3 loops. The predominan
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- Author
- Sebastian Ochsenreither; Alberto Fusi; Antonia Busse; Anne Letsch; Doreen Haase; Eckhard Thiel; Carmen Scheibenbogen; Ulrich Keilholz
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 2010
- Language
- EN
- Field
- Medicine (Health Sciences)