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Different residues in periplasmic domains of the CcmC inner membrane protein of Pseudomonas fluorescens ATCC 17400 are critical for cytochrome c biogenesis and pyoverdine-mediated iron uptake by Ahmed Gaballa; Christine Baysse; Nico Koedam; Serge Muyldermans; Pierre Cornelis is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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The inner membrane protein CcmC (CytA) of __Pseudomonas fluorescens__ ATCC17400, which has homologues in several bacteria and plant mitochondria, is needed for the biogenesis of cytochrome __c__. A CcmC‐deficient mutant is also compromised in the production and utilization of pyoverdine, the high‐affinity fluorescent siderophore. A topological model for CcmC, based on the analysis of alkaline phosphatase fusions, predicts six membrane‐spanning regions with three periplasmic loops. Site‐directed mutagenesis was used in order to assess the importance of some periplasm‐exposed residues, conserved in all CcmC homologues, for cytochrome __c__ biogenesis, and pyoverdine production/utilization. Despite the conservation of the residues His‐61, Val‐62 and Pro‐63 in the first periplasmic loop, and Leu‐184, His‐185 and Gln‐186 in the third periplasmic loop, their simultaneous replacement with Ala only partially affected cytochrome __c__ biogenesis and pyoverdine production/utilization. Simultaneous replacements of residues Trp‐115 and Gly‐116 in the second periplasmic loop substantially affected pyoverdine production/utilization but not cytochrome __c__ production. An Ala substitution of Asp‐

Who reads Different residues in periplasmic domains of the CcmC inner membrane protein of Pseudomonas fluorescens ATCC 17400 are critical for cytochrome c biogenesis and pyoverdine-mediated iron uptake?

Author

Ahmed Gaballa; Christine Baysse; Nico Koedam; Serge Muyldermans; Pierre Cornelis

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)