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Diagnostic Strategy of Metagenomic Next-generation Sequencing for Gram Negative Bacteria in Respiratory Infections by Wenyan Liang; Qun Zhang; Qian Qian; Mingyue Wang; Yuchen Ding; Ji Zhou; Yi Zhu; Yu Jin; Xuesong Chen; Hui Kong; Wei Song; Xin Lu; Xiaodong Wu; Xiaoyong Xu; Shanling Dai; Wenkui Sun is a Medicine article available to read on EtoBox.

What is Diagnostic Strategy of Metagenomic Next-generation Sequencing for Gram Negative Bacteria in Respiratory Infections about?

## Objective This study aims to identify the most effective diagnostic method for distinguishing pathogenic and non-pathogenic Gram-negative bacteria (GNB) in suspected pneumonia cases using metagenomic next-generation sequencing (mNGS) on bronchoalveolar lavage fluid (BALF) samples. ## Methods The effectiveness of mNGS was assessed on BALF samples collected from 583 patients, and the results were compared with those from microbiological culture and final clinical diagnosis. Three interpretational approaches were evaluated for diagnostic accuracy. ## Results mNGS outperformed culture significantly. Among the interpretational approaches, Clinical Interpretation (CI) demonstrated the best diagnostic performance with a sensitivity of 87.3%, specificity of 100%, positive predictive value of 100%, and negative predictive value of 98.3%. CI’s specificity was significantly higher than Simple Interpretation (SI) at 37.9%. Additionally, CI excluded some microorganisms identified as putative pathogens by SI, including __Haemophilus parainfluenzae, Haemophilus parahaemolyticus__, and __Klebsiella aerogenes__. ## Conclusion Proper interpretation of mNGS data is crucial for accurately diagnosin

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Author

Wenyan Liang; Qun Zhang; Qian Qian; Mingyue Wang; Yuchen Ding; Ji Zhou; Yi Zhu; Yu Jin; Xuesong Chen; Hui Kong; Wei Song; Xin Lu; Xiaodong Wu; Xiaoyong Xu; Shanling Dai; Wenkui Sun

Publisher

BioMed Central

Published

2024

Language

EN

Field

Medicine (Health Sciences)