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A column-switching LC/MS/ESI method for detecting tetrodotoxin and Aconitum alkaloids in serum by Makiko Hayashida; Hideyuki Hayakawa; Kentaro Wada; Takeshi Yamada; Makoto Nihira; Youkichi Ohno is a Medicine article available to read on EtoBox.

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A liquid chromatography-mass spectrometry-electrospray ionization (LC/MS/ESI) method coupled with a column-switching technique has been developed for the determination of tetrodotoxin (TTX) and Aconitum alkaloids and their metabolites, such as aconitine, mesaconitine, hypaconitine, jesaconitine, benzoylaconine, benzoylmesaconine, benzoylhypaconine and 14anisoylaconine, in serum. An on-column column-switching technique was employed to analyze TTX and Aconitum alkaloids and their metabolites without pretreatment of the serum. Combination of a multimode column with reversed phases and cation exchange for TTX, and use of a multimode column with reversed phases and a hydrophobic polymer column for Aconitum alkaloids and their metabolites provided successful separation and MS determination in ESI positive mode. A 100 ml serum sample was directly injected into a precolumn. For TTX monitored at m/z 320.1 in the selected ion monitoring mode, the calibration curve was linear within the range 0.1-100 ng/ml and the limit of detection was 0.1 ng/ml. For aconitine, mesaconitine, hypaconitine and jesaconitine, linear calibration curves were obtained up to 500 ng/ml and the limit of detection rang

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Author

Makiko Hayashida; Hideyuki Hayakawa; Kentaro Wada; Takeshi Yamada; Makoto Nihira; Youkichi Ohno

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1344-6223)

Published

2003

Language

EN

Field

Medicine (Health Sciences)