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Can I read Optical Protein Sensor for Detecting Cancer Markers in Saliva on EtoBox?
Optical Protein Sensor for Detecting Cancer Markers in Saliva by Winny Tan; Leyla Sabet; Yang Li; Tianwei Yu; Perry R. Klokkevold; David T. Wong; Chih-Ming Ho is a Engineering article available to read on EtoBox.
What is Optical Protein Sensor for Detecting Cancer Markers in Saliva about?
A surface immobilized optical protein sensor has been utilized to detect Interleukin-8 (IL-8) protein, an oral cancer marker, and can reach limit of detection (LOD) at 1.1 pM in buffer without using enzymatic amplification. Only after applying enzymatic amplification to increase the signal level by a few orders of magnitude, ELISA can reach the LOD of 1 pM level. We then develop the confocal optics based sensor for further reducing the optical noise and can extend the LOD of the surface immobilized optical protein sensor two orders in magnitude. These improvements have allowed us to detect IL-8 protein at 4.0 fM in buffer. In addition, these sensitive LODs were achieved without the use of enzymatic signal amplification, such that the simplified protocol can further facilitate the development of point-of-care devices. The ultra sensitive optical protein sensor presented in this paper has a wide number of applications in disease diagnoses. Measurements for detecting biomarkers in clinical sample are much more challenging than the measurements in buffer, due to high background noise contributed by large collections of nontarget molecules. We used clinical saliva samples to validate th
Who reads Optical Protein Sensor for Detecting Cancer Markers in Saliva?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Winny Tan; Leyla Sabet; Yang Li; Tianwei Yu; Perry R. Klokkevold; David T. Wong; Chih-Ming Ho
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)
- Published
- 2008
- Language
- EN
- Field
- Engineering (Physical Sciences)