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The Zea mays mutants opaque-2 and opaque-7 disclose extensive changes in endosperm metabolism as revealed by protein, amino acid, and transcriptome-wide analyses by Hans Hartings; Massimiliano Lauria; Nadia Lazzaroni; Raul Pirona; Mario Motto is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Background The changes in storage reserve accumulation during maize (__Zea mays__ L.) grain maturation are well established. However, the key molecular determinants controlling carbon flux to the grain and the partitioning of carbon to starch and protein are more elusive. The __Opaque-2__ (__O2__) gene, one of the best-characterized plant transcription factors, is a good example of the integration of carbohydrate, amino acid and storage protein metabolisms in maize endosperm development. Evidence also indicates that the __Opaque-7__ (__O7__) gene plays a role in affecting endosperm metabolism. The focus of this study was to assess the changes induced by the __o2__ and __o7__ mutations on maize endosperm metabolism by evaluating protein and amino acid composition and by transcriptome profiling, in order to investigate the functional interplay between these two genes in single and double mutants. ## Results We show that the overall amino acid composition of the mutants analyzed appeared similar. Each mutant had a high Lys and reduced Glx and Leu content with respect to wild type. Gene expression profiling, based on a unigene set composed of 7,250 ESTs, allowed us to identify a ser

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Author

Hans Hartings; Massimiliano Lauria; Nadia Lazzaroni; Raul Pirona; Mario Motto

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2000-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-2164)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)