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MDM2-P53 Signaling Pathway-Mediated Upregulation of CDC20 Promotes Progression of Human Diffuse Large B-Cell Lymphoma by Sun, Chengtao; Li, Mengzhen; Feng, Yanfen; Sun, Feifei; Zhang, Li; Xu, Yanjie; Lu, Suying; Zhu, Jia; Huang, Junting; Wang, Juan; Hu, Yang; Zhang, Yizhuo is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is MDM2-P53 Signaling Pathway-Mediated Upregulation of CDC20 Promotes Progression of Human Diffuse Large B-Cell Lymphoma about?

## Background Cell-division cycle 20 (CDC20) is overexpressed in a variety of tumor cells and is negatively regulated by wild-type p53 (wtp53). Our previous study uncovered that CDC20 was upregulated and associated with poor outcome in diffuse large B-cell lymphoma (DLBCL) based on bioinformatics analysis. Dysregulation of the MDM2-p53 is a major mechanism to promote DLBCL. Thus, we hypothesized that CDC20 could be a downstream gene of the MDM2-p53 signaling pathway. However, the clinical significance and mechanistic role of a novel MDM2-p53-CDC20 signaling pathway in DLBCL have still remained unclear. ## Materials and Methods RT-qPCR was performed in MDM2 knocked down (KD) and control (Ctrl) OCI-Ly3/OCI-Ly10 cells to investigate whether CDC20 was a downstream gene of the MDM2-p53 pathway. The effects of CDC20 on cell proliferation, cell cycle and apoptosis were assessed, as well as the role of CDC20 in suppressing tumorigenicity in vivo. Furthermore, we also investigated the roles of CDC20 and MDM2 in progression of DLBCL and the underlying mechanisms. ## Results The results of RT-qPCR revealed that CDC20 was downregulated while TP53 was upregulated in MDM2 KD OCI-Ly3 and OCI-Ly10

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Author

Sun, Chengtao; Li, Mengzhen; Feng, Yanfen; Sun, Feifei; Zhang, Li; Xu, Yanjie; Lu, Suying; Zhu, Jia; Huang, Junting; Wang, Juan; Hu, Yang; Zhang, Yizhuo

Publisher

Dove Press Ltd.; Dove Medical Press; Dove Medical Press Ltd; [Auckland N.Z.]: Dove Medical Press, c2008-; Informa UK Limited; Dove Medical Press Ltd. (ISSN 1178-6930)

Published

2020

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)