Opening book details…
Can I read Directly induced human retinal ganglion cells mimic fetal RGCs and are neuroprotective after transplantation in vivo on EtoBox?
Directly induced human retinal ganglion cells mimic fetal RGCs and are neuroprotective after transplantation in vivo by Ziming Luo; Kun-Che Chang; Suqian Wu; Catalina Sun; Xin Xia; Michael Nahmou; Minjuan Bian; Rain R. Wen; Ying Zhu; Sahil Shah; Bogdan Tanasa; Marius Wernig; Jeffrey L. Goldberg is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Directly induced human retinal ganglion cells mimic fetal RGCs and are neuroprotective after transplantation in vivo about?
## Summary Retinal ganglion cell (RGC) replacement therapy could restore vision in glaucoma and other optic neuropathies. We developed a rapid protocol for directly induced RGC (iRGC) differentiation from human stem cells, leveraging overexpression of NGN2. Neuronal morphology and neurite growth were observed within 1 week of induction; characteristic RGC-specific gene expression confirmed identity. Calcium imaging demonstrated γ-aminobutyric acid (GABA)-induced excitation characteristic of immature RGCs. Single-cell RNA sequencing showed more similarities between iRGCs and early-stage fetal human RGCs than retinal organoid-derived RGCs. Intravitreally transplanted iRGCs survived and migrated into host retinas independent of prior optic nerve trauma, but iRGCs protected host RGCs from neurodegeneration. These data demonstrate rapid iRGC generation __in vitro__ into an immature cell with high similarity to human fetal RGCs and capacity for retinal integration after transplantation and neuroprotective function after optic nerve injury. The simplicity of this system may benefit translational studies on human RGCs.
Who reads Directly induced human retinal ganglion cells mimic fetal RGCs and are neuroprotective after transplantation in vivo?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ziming Luo; Kun-Che Chang; Suqian Wu; Catalina Sun; Xin Xia; Michael Nahmou; Minjuan Bian; Rain R. Wen; Ying Zhu; Sahil Shah; Bogdan Tanasa; Marius Wernig; Jeffrey L. Goldberg
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)