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Sputum microbiome profiling in COPD: beyond singular pathogen detection by Ditz, Benedikt; Christenson, Stephanie; Rossen, John; Brightling, Chris; Kerstjens, Huib A M; van den Berge, Maarten; Faiz, Alen is a Medicine article available to read on EtoBox.

What is Sputum microbiome profiling in COPD: beyond singular pathogen detection about?

Culture-independent microbial sequencing techniques have revealed that the respiratory tract harbours a complex microbiome not detectable by conventional culturing methods. The contribution of the microbiome to chronic obstructive pulmonary disease (COPD) pathobiology and the potential for microbiome-based clinical biomarkers in COPD are still in the early phases of investigation. Sputum is an easily obtainable sample and has provided a wealth of information on COPD pathobiology, and thus has been a preferred sample type for microbiome studies. Although the sputum microbiome likely reflects the respiratory microbiome only in part, there is increasing evidence that microbial community structure and diversity are associated with disease severity and clinical outcomes, both in stable COPD and during the exacerbations. Current evidence has been limited to mainly cross-sectional studies using 16S rRNA gene sequencing, attempting to answer the question ‘who is there?’ Longitudinal studies using standardised protocols are needed to answer outstanding questions including differences between sputum sampling techniques. Further, with advancing technologies, microbiome studies are shifting be

Who reads Sputum microbiome profiling in COPD: beyond singular pathogen detection?

Author

Ditz, Benedikt; Christenson, Stephanie; Rossen, John; Brightling, Chris; Kerstjens, Huib A M; van den Berge, Maarten; Faiz, Alen

Publisher

BMJ Publishing Group; BMJ (ISSN 0040-6376)

Published

2020

Language

EN

Field

Medicine (Health Sciences)