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Radioimmunoassay (RIA) is a technique introduced in 1960 that uses the specificity of antibody-antigen binding and radioactivity to quantify proteins in a mixture. It revolutionized research and clinical practice by allowing detection of hormone levels in blood. RIA involves mixing a radioactive antigen with antibodies and unlabeled antigen samples, then separating bound from unbound antigens and measuring radioactivity levels to determine concentrations. Enzyme-linked immunosorbent assay (ELISA) is a widel
- Author
- Vinayak Chura
- Language
- EN