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This document describes the use of a CarboPac PA200 column for determining plant-derived neutral oligo- and polysaccharides using high-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD). The PA200 column provides improved resolution compared to earlier columns and requires lower concentrations of sodium acetate eluent. The document outlines the equipment, reagents, standards, chromatography conditions, eluent and sample preparation for separating oligo- and polysaccharid

Author

juakfuenmayor

Language

EN