Can I read Opposite expression of securin and γ‐H2AX regulates baicalein‐induced cancer cell death on EtoBox?

Opposite expression of securin and γ‐H2AX regulates baicalein‐induced cancer cell death by Ren‐Huei Jiang; Wen‐Chi Su; Huei‐Fang Liu; Hou‐Syun Huang; Jui‐I Chao is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Opposite expression of securin and γ‐H2AX regulates baicalein‐induced cancer cell death about?

## Abstract Securin and γ‐H2AX have been shown to regulate cell survival and genomic stability. However, it is still unknown how the expression and regulation of these proteins is altered following treatment with baicalein, a natural flavonoid extracted from the __Scutellaria baicalensis__ root. In the present study, we investigate the possible roles of securin and γ‐H2AX in baicalein‐induced cancer cell death. Baicalein reduced cell viability in a variety of human cancer cell lines, including bladder, cervical, colon, and lung cancer cells. Interestingly, baicalein treatment (40–80 μM for 24 h) markedly inhibited securin expression, while the levels of γ‐H2AX were elevated. Abnormal spindle formation and chromosomal segregation were induced by baicalein. Furthermore, wild type HCT116 cancer cells had a higher incidence of cytotoxicity and apoptosis than securin‐null HCT116 cells following treatment with baicalein. In contrast, baicalein increased the levels of γ‐H2AX to a similar extent in both cell types. Transfection with H2AX siRNA further increased baicalein‐induced cell death. Additionally, blockade of the AKT pathway by treatment with wortmannin or AKT shRNA lowered the leve

Who reads Opposite expression of securin and γ‐H2AX regulates baicalein‐induced cancer cell death?

Author

Ren‐Huei Jiang; Wen‐Chi Su; Huei‐Fang Liu; Hou‐Syun Huang; Jui‐I Chao

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

2010

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)