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Identification of signaling motifs within human Fc gamma RIIa and Fc gamma RIIb isoforms by Van den Herik-Oudijk, IE; Capel, PJ; van der Bruggen, T; Van de Winkel, JG is a Medicine article available to read on EtoBox.

What is Identification of signaling motifs within human Fc gamma RIIa and Fc gamma RIIb isoforms about?

To assess the functional capacity of the heterogeneous Fc gamma RII (CD32) family and to identify critical regions for functioning, we generated a panel of B-cell transfectants. The Fc gamma R-negative B-cell line IIA1.6 was transfected with wild-type or mutant human Fc gamma RIIa and IIb molecules. Solely Fc gamma RIIa-expressing IIA1.6 cells were capable of phagocytosing opsonized Staphylococcus aureus bacteria, and cross-linking of Fc gamma RIIa triggered a rapid induction of tyrosine phosphorylation after 20 seconds. Analysis of Fc gamma RIIa mutants identified the immunoreceptor tyrosine-based activation motif (ITAM; previously described as ARH-1 motif) within the IIa cytoplasmic tail to be critical for B-cell activation. In contrast, Fc gamma RIIb isoforms triggered tyrosine phosphorylation on cross-linking with much slower kinetics (> 3 minutes) than Fc gamma RIIa. Furthermore, solely Fc gamma RIIb molecules proved capable of downregulating [Ca2+]i and interleukin-2 production on co-cross-linking with sIgG in IIA1.6. The Fc gamma RIIb-mediated functions were absent in Fc gamma RIIb mutants in which the tyrosine or leucine within the YSLL motif in a conserved 13-aa region (no

Who reads Identification of signaling motifs within human Fc gamma RIIa and Fc gamma RIIb isoforms?

Author

Van den Herik-Oudijk, IE; Capel, PJ; van der Bruggen, T; Van de Winkel, JG

Publisher

American Society of Hematology (ISSN 0006-4971)

Published

1995

Language

EN

Field

Medicine (Health Sciences)