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Can I read Laccase Immobilization on Enzymatically Functionalized Polyamide 6,6 Fibres on EtoBox?
Laccase Immobilization on Enzymatically Functionalized Polyamide 6,6 Fibres by Carla Silva; Carla Joana Silva; Andrea Zille; Georg M. Guebitz; Artur Cavaco-Paulo is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Laccase Immobilization on Enzymatically Functionalized Polyamide 6,6 Fibres about?
Polyamide matrices, such as membranes, gels and non-wovens, have been applied as supports for enzyme immobilization, although in literature the enzyme immobilization on woven nylon matrices is rarely reported. In this work, a protocol for a Trametes hirsuta laccase immobilization using woven polyamide 6,6 (nylon) was developed. A 2 4 full factorial design was used to study the influence of pH, spacer (1,6-hexanediamine), enzyme and crosslinker concentration on the efficiency of immobilization. The factors enzyme dosage and spacer seem to have played a critical role in the immobilization of laccase onto nylon support. Under optimized working conditions (29 U mL -1 of laccase, 10% of glutaraldehyde, pH = 5.5, with the presence of the spacer), the half-life time attained was about 78 h (18% higher than that of free enzyme), the protein retention was 30% and the immobilization yield was 2%. The immobilized laccase has potential for application in the continuous decolourization of textile effluents, where it can be applied into a membrane reactor.
Who reads Laccase Immobilization on Enzymatically Functionalized Polyamide 6,6 Fibres?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Carla Silva; Carla Joana Silva; Andrea Zille; Georg M. Guebitz; Artur Cavaco-Paulo
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 0141-0229)
- Published
- 2007
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)