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Stored mRNA in cotyledons of Vigna unguiculata seeds: nucleotide sequence of cloned cDNA for a stored mRNA and induction of its synthesis by precocious germination by Nobuhiro Ishibashi; Daisuke Yamauchi; Takao Minamikawa is a Agricultural and Biological Sciences article available to read on EtoBox.
What is Stored mRNA in cotyledons of Vigna unguiculata seeds: nucleotide sequence of cloned cDNA for a stored mRNA and induction of its synthesis by precocious germination about?
By differential hybridization screening, we previously selected a class of cDNA clones from a 2gtl0 cDNA library that was constructed from the total poly(A) + RNA of mature cowpea cotyledons (Plant Cell Physiol 31: 39-44, 1990). pSAS10, a clone of this class, hybridized with a cDNA probe complementary to poly(A) + RNA from cotyledons collected 1 day after the onset of imbibition (DAI), but not with the cDNA probe from cotyledons at developmental stage II (13 to 15 days after flowering, DAF). pSAS 10 mRNA was detectable only in cotyledons at developmental stage III (17 to 19 DAF) or later, and its level began to decline when seeds germinated. We have suggested that pSAS 10 mRNA is likely to belong to the class of'stored' mRNA or the mRNA that is formed at the late stage of seed maturation, is conserved in quiescent seeds and becomes functional at the early stage of germination. We determined the nucleotide sequence of p SAS 10 cDNA consisting of 459 bp and an approximately 36 bp poly(A) tract, and deduced the amino acid sequence of its product, a 10-kDa cysteine-rich polypeptide. Synthesis of pSAS 10 mRNA was induced just before germination began, not only in mature seeds but also i
Who reads Stored mRNA in cotyledons of Vigna unguiculata seeds: nucleotide sequence of cloned cDNA for a stored mRNA and induction of its synthesis by precocious germination?
It is typically read by researchers, students, and practitioners in Agricultural and Biological Sciences.
- Author
- Nobuhiro Ishibashi; Daisuke Yamauchi; Takao Minamikawa
- Publisher
- Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 0167-4412)
- Published
- 1990
- Language
- EN
- Field
- Agricultural and Biological Sciences (Life Sciences)