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Ultra-sensitive Biosensor Based on Mesocellular Silica Foam for Organophosphorous Pesticide Detection by Shuo Wu; Lili Zhang; Lin Qi; Shengyang Tao; Xiaoqin Lan; Zhiguang Liu; Changgong Meng is a Engineering article available to read on EtoBox.
What is Ultra-sensitive Biosensor Based on Mesocellular Silica Foam for Organophosphorous Pesticide Detection about?
A sensitive amperometric acetylcholinesterase (AChE) biosensor was fabricated based on mesocellular silica foam (MSF), which functioned as both an enzyme immobilization matrix and a solid phase extraction (SPE) material for the preconcentration of target molecules. The hydrophilic interface, the good mechanical/chemical stability, and the suitable pore dimension of MSF provided the entrapped AChE a good environment to well maintain its bioactivity at basic condition. The AChE immobilized in MSF showed improved catalytic ability for the hydrolysis of acetylthiocholine, as evidenced by the increasing of the oxidation current of thiocholine, the enzymatic catalytic hydrolysis production of acetylthiocholine. In addition, the MSF with large surface area showed a modest adsorption capacity for monocrotophos, a model organophosphate used in this study, via the hydrogen bond or physical adsorption interaction. The combination of the SPE and the good enzyme immobilization ability in MSF significantly promoted the sensitivity of the biosensor, and the limit of detection has lowered to 0.05 ng/mL. The biosensor exhibited accuracy, good reproducibility, and acceptable stability when used for
Who reads Ultra-sensitive Biosensor Based on Mesocellular Silica Foam for Organophosphorous Pesticide Detection?
It is typically read by researchers, students, and practitioners in Engineering.
- Author
- Shuo Wu; Lili Zhang; Lin Qi; Shengyang Tao; Xiaoqin Lan; Zhiguang Liu; Changgong Meng
- Publisher
- Elsevier Science; Elsevier ; Elsevier Ltd.; Elsevier BV (ISSN 0956-5663)
- Published
- 2011
- Language
- EN
- Field
- Engineering (Physical Sciences)