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Involvement of cathepsin B in the processing and secretion of interleukin-1β in chromogranin A-stimulated microglia by Kayo Terada; Jun Yamada; Yoshinori Hayashi; Zhou Wu; Yasuo Uchiyama; Christoph Peters; Hiroshi Nakanishi is a Neuroscience article available to read on EtoBox.
What is Involvement of cathepsin B in the processing and secretion of interleukin-1β in chromogranin A-stimulated microglia about?
## Abstract Cathepsin B (CB) is a cysteine lysosomal protease implicated in a number of inflammatory diseases. Although it is now evident that caspase‐1, an essential enzyme for maturation of interleukin‐1β (IL‐1β), can be activated through the inflammasome, there is still evidence suggesting the existence of lysosomal‐proinflammatory caspase pathways. In the present study, a marked induction of pro‐IL‐1β, its processing to the mature form and secretion were observed in the primary cultured microglia prepared from wild‐type mice after stimulation with chromogranin A (CGA). Although pro‐IL‐1β also markedly increased in microglia prepared from CB‐deficient mice, CB‐deficiency abrogated the pro‐IL‐1β processing. CA‐074Me, a specific inhibitor for CB, inhibited the pro‐IL‐1β maturation and its release from microglia. Furthermore, the caspase‐1 activation was also inhibited by CA‐074Me and E‐64d, a broad cysteine protease inhibitor. After treatment with CGA, CB was markedly induced at both protein and mRNA levels. The induced pro‐CB was rapidly processed to its mature form. The immunoreactivity for CB co‐localized with both that for caspase‐1 and the cleaved IL‐1β, in the acidic enlarge
Who reads Involvement of cathepsin B in the processing and secretion of interleukin-1β in chromogranin A-stimulated microglia?
It is typically read by researchers, students, and practitioners in Neuroscience.
- Author
- Kayo Terada; Jun Yamada; Yoshinori Hayashi; Zhou Wu; Yasuo Uchiyama; Christoph Peters; Hiroshi Nakanishi
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)
- Published
- 2009
- Language
- EN
- Field
- Neuroscience (Life Sciences)