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Validation of a Liquid Chromatography–tandem Mass Spectrometric Assay for the Quantitative Determination of Hydrastine and Berberine in Human Serum by Prem K. Gupta; Martha Hubbard; Bill Gurley; Howard P. Hendrickson is a Chemistry article available to read on EtoBox.

What is Validation of a Liquid Chromatography–tandem Mass Spectrometric Assay for the Quantitative Determination of Hydrastine and Berberine in Human Serum about?

A high throughput liquid chromatography/tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of berberine and hydrastine in human serum, after oral administration of goldenseal (Hydrastis canadensis L.), was developed using simple acetonitrile treatment of serum samples. Noscapine served as the internal standard. Lower limit of quantification for both analytes was 0.1 ng mL -1 using positive ion electrospray tandem mass spectrometry (MS/MS). The intra-day (n = 5) accuracy and precision of the method for hydrastine was 82 ± 8.8%, 97.9 ± 2.4% and 96.2 ± 3.3%, respectively. The inter-day (n = 4) accuracy and precision for hydrastine was 90.0 ± 15.17%, 99.9 ± 7.1% and 98 ± 6.54%, respectively. For berberine quantitation intra-day accuracy and precision was 96.0 ± 8.4%, 92.5 ± 4.7% and 94.4 ± 3.7%, respectively. The respective values for inter-day quantitation were 91.0 ± 8.4%, 94.3 ± 4.7% and 94.4 ± 3.7%. The analytical recovery for hydrastine was 82.4-96.2% and for berberine it was 94.4-96.0%. The analytes and noscapine were stable for 24 h at room temperature (CV 5-10%). Matrix ion effects were studied by post-column infusion of hydrastine and berberine, calc

Who reads Validation of a Liquid Chromatography–tandem Mass Spectrometric Assay for the Quantitative Determination of Hydrastine and Berberine in Human Serum?

Author

Prem K. Gupta; Martha Hubbard; Bill Gurley; Howard P. Hendrickson

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0731-7085)

Published

2009

Language

EN

Field

Chemistry (Physical Sciences)