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In Vivo Imaging of Fluorescent Albumin Modified with Pyruvylated-human-type Complex Oligosaccharide Reveals Sialylation-like Biodistribution and Kinetics by Ryoichiro Fukuhara; Akihiro Ogura; Sho Yoshinaga; Takamasa Fukunaga; Takashi Kinoshita; Wataru Sumiyoshi; Yujiro Higuchi; Katsunori Tanaka; Kaoru Takegawa is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is In Vivo Imaging of Fluorescent Albumin Modified with Pyruvylated-human-type Complex Oligosaccharide Reveals Sialylation-like Biodistribution and Kinetics about?
Both pyruvylation and sialylation onto the terminus of oligosaccharides of N-glycoproteins seem to be structurally and functionally similar with a property of conferring negative charge. However, detailed molecular characteristics of pyruvylation and sialylation in vivo were elusive. Here, to investigate an effect of terminal pyruvylation to N-glycan on in vivo biodistribution and kinetics, we prepared human serum albumin (HSA) modified with pyruvylated N-glycan (PvG), conjugated with HiLyte Fluor 750 (FL750-PvGHSA). In vivo imaging by using FL750-PvGHSA revealed that terminally pyruvylated N-glycoalbumin was excreted like sialylated N-glycoalbumin, suggesting that pyruvylation mimics sialylation in in vivo biodistribution and kinetics of N-glycoproteins. Terminal pyruvylation onto N-glycans can be a potential tool for a novel glycoengineering strategy.
Who reads In Vivo Imaging of Fluorescent Albumin Modified with Pyruvylated-human-type Complex Oligosaccharide Reveals Sialylation-like Biodistribution and Kinetics?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ryoichiro Fukuhara; Akihiro Ogura; Sho Yoshinaga; Takamasa Fukunaga; Takashi Kinoshita; Wataru Sumiyoshi; Yujiro Higuchi; Katsunori Tanaka; Kaoru Takegawa
- Publisher
- Elsevier BV
- Published
- 2022
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)