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Genomic and proteomic characterization of Staphylococcus aureus mastitis isolates of bovine origin by Carmen Wolf; Harald Kusch; Stefan Monecke; Dirk Albrecht; Silva Holtfreter; Christof von Eiff; Wolfram Petzl; Pascal Rainard; Barbara M. Bröker; Susanne Engelmann is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Genomic and proteomic characterization of Staphylococcus aureus mastitis isolates of bovine origin about?
## Abstract __Staphylococcus aureus__ colonizes and infects humans as well as animals. In the present study, 17 __S. aureus__ strains isolated from cows suffering from mastitis were characterized. The well‐established multilocus sequence typing (MLST) technique and a diagnostic microarray covering 185 __S. aureus__ virulence and resistance genes were used for genetic and epidemiological analyses. Virulence gene expression studies were performed by analyzing the extracellular protein pattern of each isolate on 2‐D gels. By this way, a pronounced heterogeneity of the extracellular proteome between the bovine isolates has been observed which was attributed to genome plasticity and variation of gene expression. Merely 12 proteins were expressed in at least 80% of the isolates, i.e. Atl, Aur, GlpQ, Hla, LtaS, Nuc, PdhB, SAB0846, SAB2176, SAB0566, SspA, and SspB forming the core exoproteome. Fifteen extracellular proteins were highly variably expressed and only present in less than 20% of the isolates. This includes the serine proteases SplB, C, and F, and the superantigens SEC‐bov, SEL and TSST‐1. Compared to human isolates we identified at least six proteins with significantly differen
Who reads Genomic and proteomic characterization of Staphylococcus aureus mastitis isolates of bovine origin?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Carmen Wolf; Harald Kusch; Stefan Monecke; Dirk Albrecht; Silva Holtfreter; Christof von Eiff; Wolfram Petzl; Pascal Rainard; Barbara M. Bröker; Susanne Engelmann
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley; Research Square (ISSN 1615-9853)
- Published
- 2011
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)