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Selection and characterization of toxic Aspergillus spore-specific DNA aptamer using spore-SELEX by Jin-Woo Seo; Jee Young Kim; Da Hee Kim; Jeong-Joo Oh; Young Jun Kim; Gyu-Hyeok Kim is a Materials Science article available to read on EtoBox.

What is Selection and characterization of toxic Aspergillus spore-specific DNA aptamer using spore-SELEX about?

As airborne spores of toxic __Aspergillus__ species cause mild symptoms to invasive fungal infections, their indoor concentration should be controlled through real-time management. Aptamer-based biosensors could provide economical and simple solutions for point-of-care. In this study, we isolated aptamers binding to the spores of three representative toxic __Aspergillus__ species (__A. fumigatus__, __A. flavus__, and __A. niger__) for the first time, using cell-SELEX (systematic evolution of ligands through exponential enrichment). Among the aptamer candidates, Asp-3 showed a broad and high binding affinity for the __Aspergillus__ spores. Considering the low binding affinity with proteinase-treated spores, we speculated that the Asp-3 binding sites could be possibly associated with cell surface proteins. The high Asp-3 specificity was confirmed by comparing the binding affinity between the __Aspergillus__ target species and other common indoor fungal species. Moreover, we also established quantitative linear relationships between Asp-3 and the spore concentration of each __Aspergillus__ species. Therefore, the selected Asp-3 aptamer, conjugated with detection sensors, could be an e

Who reads Selection and characterization of toxic Aspergillus spore-specific DNA aptamer using spore-SELEX?

Author

Jin-Woo Seo; Jee Young Kim; Da Hee Kim; Jeong-Joo Oh; Young Jun Kim; Gyu-Hyeok Kim

Published

2021

Language

EN

Field

Materials Science (Physical Sciences)