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Inhibition of Collagenase Breakdown of Equine Corneas by Tetanus Antitoxin, Equine Serum and Acetylcysteine by John C. Haffner; Kellie A. Fecteau; Hugo Eiler is a Medicine article available to read on EtoBox.

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## Abstract **Objective** To determine whether tetanus antitoxin, equine serum, and acetylcysteine, which are currently used in the treatment of equine corneal ulcer, inhibit the digestion of equine corneal collagen when exposed to collagenase __in vitro__. **Animals studied** Corneas from 40 adult horses. **Procedures** Sections of equine corneas were incubated with saline, a solution of bacterial collagenase in saline, bacterial collagenase in saline plus equine tetanus antitoxin, bacterial collagenase in saline plus equine serum, or bacterial collagenase in saline plus acetylcysteine. Each one of the collagenase inhibitors was tested at different concentrations. The degree of corneal collagen digestion was determined by concentrations of hydroxyproline released into the incubation media and/or by weight loss of the cornea. **Results** Corneas exposed to collagenase released a significant (0.05 level) large amount of hydroxyproline (43.1 ± 2.3 μg/mL/100 mg cornea/5 h) and decreased cornea weight by up to 89%. Blood serum (200 μL/mL), purified albumin or globulin fractions of serum, tetanus antitoxin (120 units/mL), and acetylcysteine (20 mg/mL) when used at the highest concentrat

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Author

John C. Haffner; Kellie A. Fecteau; Hugo Eiler

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1463-5216)

Published

2003

Language

EN

Field

Medicine (Health Sciences)