Can I read Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization on EtoBox?

Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization by Markiyan Oliynyk; Christian B. W. Stark; Apoorva Bhatt; Michelle A. Jones; Zoë A. Hughes-Thomas; Christopher Wilkinson; Zoryana Oliynyk; Yuliya Demydchuk; James Staunton; Peter F. Leadlay is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization about?

## Summary The analysis of a candidate biosynthetic gene cluster (97 kbp) for the polyether ionophore monensin from __Streptomyces cinnamonensis__ has revealed a modular polyketide synthase composed of eight separate multienzyme subunits housing a total of 12 extension modules, and flanked by numerous other genes for which a plausible function in monensin biosynthesis can be ascribed. Deletion of essentially all these clustered genes specifically abolished monensin production, while overexpression in __S. cinnamonensis__ of the putative pathway‐specific regulatory gene __monR__ led to a fivefold increase in monensin production. Experimental support is presented for a recently‐proposed mechanism, for oxidative cyclization of a linear polyketide intermediate, involving four enzymes, the products of __monBI__, __monBII__, __monCI__ and __monCII__. In frame deletion of either of the individual genes __monCII__ (encoding a putative cyclase) or __monBII__ (encoding a putative novel isomerase) specifically abolished monensin production. Also, heterologous expression of __monCI__, encoding a flavin‐linked epoxidase, in __S. coelicolor__ was shown to significantly increase the ability of __

Who reads Analysis of the biosynthetic gene cluster for the polyether antibiotic monensin in Streptomyces cinnamonensis and evidence for the role of monB and monC genes in oxidative cyclization?

Author

Markiyan Oliynyk; Christian B. W. Stark; Apoorva Bhatt; Michelle A. Jones; Zoë A. Hughes-Thomas; Christopher Wilkinson; Zoryana Oliynyk; Yuliya Demydchuk; James Staunton; Peter F. Leadlay

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0950-382X)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)