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Hydrodynamics‐based transfection of the liver: entrance into hepatocytes of DNA that causes expression takes place very early after injection by Fanjam Andrianaivo; Michèle Lecocq; Simone Wattiaux‐De Coninck; Robert Wattiaux; Michel Jadot is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Hydrodynamics‐based transfection of the liver: entrance into hepatocytes of DNA that causes expression takes place very early after injection about?
## Abstract ## Background The mechanism of gene transfer into hepatocytes by the hydrodynamics‐based transfection procedure is not clearly understood. It has been shown that, after a hydrodynamic injection, a large proportion of plasmid DNA remains intact in the liver where it is bound to plasma membrane and suggested that this DNA could be responsible for the efficiency of the transfection. ## Methods We have investigated the problem by giving mice a hydrodynamic injection of isotonic NaCl, followed at different time intervals by a conventional injection of DNA, cold or labelled with ^35^S, with cDNA of luciferase as a reporter gene. Then, we determined the consequences of that dual injection on luciferase expression and on DNA uptake by the liver and its intracellular fate. By such experiments, it is possible to establish the time dependency of the induction of liver changes caused by a hydrodynamic injection on the one hand and the expression and DNA uptake and fate on the other. Moreover, some experiments have been performed on primary cultures of hepatocytes isolated after a hydrodynamic injection of DNA. ## Results When DNA is given to mice by a conventional injection a few s
Who reads Hydrodynamics‐based transfection of the liver: entrance into hepatocytes of DNA that causes expression takes place very early after injection?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Fanjam Andrianaivo; Michèle Lecocq; Simone Wattiaux‐De Coninck; Robert Wattiaux; Michel Jadot
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1099-498X)
- Published
- 2004
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)