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Development of a 5'-nuclease PCR assay for detecting Shiga toxin-producing Escherichia coli O145 based on the identification of an 'O-island 29' homologue by S. Perelle; F. Dilasser; J. Grout; P. Fach is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Development of a 5'-nuclease PCR assay for detecting Shiga toxin-producing Escherichia coli O145 based on the identification of an 'O-island 29' homologue about?

## Aims: A dna sequence, from escherichia coli stec o145, homologous to o-island 29 from stec o157 is described, together with a real-time pcr assay for detecting it. ## Methods and results: Pcr and sequencing were used to identify the 'o-island 29' homologous dna sequence from stec o145 (strain vth34). the sequence divergence between the stec o145 and o157 'o-island 29' allowed a stec o145 5'-nuclease pcr assay to be developed. ## Conclusions: The characterization of a novel locus in stec o145 has allowed a specific o145 serogroup 5'-nuclease pcr assay to be designed. ## Significance and impact of the study: These findings increase the number of serogroup pcr assays available as alternatives to classical o-serotyping of e. coli.

Who reads Development of a 5'-nuclease PCR assay for detecting Shiga toxin-producing Escherichia coli O145 based on the identification of an 'O-island 29' homologue?

Author

S. Perelle; F. Dilasser; J. Grout; P. Fach

Publisher

Oxford University Press (OUP)

Published

2003

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)