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Recombinant DNA in Human Insulin Production by Cleo Poulos is a document available to read on EtoBox.

What is Recombinant DNA in Human Insulin Production about?

1. mRNA from human insulin-producing cells is used to make cDNA, which is then amplified using PCR. 2. The insulin gene is isolated from the cDNA and inserted into a bacterial plasmid using restriction enzymes and DNA ligase. 3. The plasmid containing the human insulin gene is then inserted into E. coli bacteria, which are grown in fermenters to produce human insulin identical to natural insulin.

Author

Cleo Poulos

Language

EN