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In vitro and In vivo studies of local arterial gene delivery and transfection using lipopolyplexes-embedded stents by Luis A. Brito; Saradha Chandrasekhar; Steven R. Little; Mansoor M. Amiji is a Engineering article available to read on EtoBox.

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## Abstract Gene‐eluting stents can have profound impact in the treatment of coronary restenosis, especially when the encoded protein can re‐endothelialize the arterial lumen. In this study, we have examined gene delivery __in vitro__ and __in vivo__ using poly(beta‐amino ester) (PbAE) precondensed plasmid DNA‐containing cationic liposomes or lipopolyplexes (LPP) immobilized on stainless steel meshes and stents using gelatin coatings. __In vitro__ studies using LPP‐immobilized on 50 mm round meshes using type A and B gelatin coatings showed that LPP were efficiently internalized in human aortic smooth muscle cells (SMC) over time, leading to green fluorescent protein (GFP) expression. Type B gelatin coating was found to be more effective in intracellular delivery and transgene expression efficiency and, as such, was used for stent coating. __In vivo__ studies, carried out in iliac artery restenosis model in New Zealand white rabbits, also showed GFP expression in arterial tissues after 24 h of implantation. Based on these encouraging preliminary results, LPP‐based formulations can serve as a safe and effective nonviral gene delivery system for effective treatment of coronary resten

Who reads In vitro and In vivo studies of local arterial gene delivery and transfection using lipopolyplexes-embedded stents?

Author

Luis A. Brito; Saradha Chandrasekhar; Steven R. Little; Mansoor M. Amiji

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2009

Language

EN

Field

Engineering (Physical Sciences)