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Interferon-γ Sensitizes Osteosarcoma Cells to Fas-induced Apoptosis by Up-regulating Fas Receptors and Caspase-8 by Hiroto Inaba; Maria Glibetic; Steven Buck; Yaddanapudi Ravindranath; Joseph Kaplan is a Medicine article available to read on EtoBox.

What is Interferon-γ Sensitizes Osteosarcoma Cells to Fas-induced Apoptosis by Up-regulating Fas Receptors and Caspase-8 about?

## Abstract ## Background Osteosarcoma is the third most frequent neoplasm in adolescents. Although chemotherapy, frequently used in pre‐ and post‐operative settings, has resulted in significant improvement in disease‐free survival, some patients show little sensitivity to chemotherapy and alternative therapeutic strategies are needed. Because the Fas ligand/Fas receptor (CD95, APO‐1) apoptosis pathway is a potential therapeutic target in osteosarcomas, we examined the effect of IFN‐γ on Fas‐induced apoptosis in four osteosarcoma cell lines. ## Procedure and Results As measured by flow cytometry, all cell lines expressed cell surface IFN‐γ receptors, and when cultured for 2 days in the presence of IFN‐γ, all cell lines exhibited a significant increase in expression of Fas receptors. By flow cytometric detection of intracellular fragmented DNA as a marker of apoptosis, all cell lines cultured with either IFN‐γ or anti‐Fas antibody (clone CH‐11) alone showed only moderate apoptosis, whereas significantly high levels of apoptosis occurred in cells cultured with both IFN‐γ and CH‐11. Western blotting analysis also revealed that IFN‐γ caused up‐regulation of caspase‐8 in all cell lines,

Who reads Interferon-γ Sensitizes Osteosarcoma Cells to Fas-induced Apoptosis by Up-regulating Fas Receptors and Caspase-8?

Author

Hiroto Inaba; Maria Glibetic; Steven Buck; Yaddanapudi Ravindranath; Joseph Kaplan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1545-5009)

Published

2004

Language

EN

Field

Medicine (Health Sciences)