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The purification of CD34^+^ cells from human cord blood: comparison of separation techniques and cytokine requirements for optimal growth of clonogenic progenitors by PIERRE CHARBORD; ISOBEL NEWTON; LAURENT VOILLAT; JEAN PATRICK SCHAAL; PATRICK HERVE is a Medicine article available to read on EtoBox.
What is The purification of CD34^+^ cells from human cord blood: comparison of separation techniques and cytokine requirements for optimal growth of clonogenic progenitors about?
This study was performed to assess the methods which gave maximal recovery of purified CD34/45^+^ cells from a cord blood specimen and optimal growth of progenitors cultured from the purified cells. Cord blood samples were separated using Percoll gradients (either one (1.080) or two successive (1.080 and 1.068) gradient(s)) and commercially available devices for CD34^+^ cell isolation (affinity columns as manufactured by CellPro Inc. or immunomagnetic separation procedure as devised by Baxter Inc.). ‘CellPro’ or ‘Baxter’ techniques gave similar results in terms of nucleated, CD34/45^+^ and progenitor cell concentration; however, the yield of CD34/45^+^ cells in the CD34^+^ enriched fraction was significantly higher when using the ‘CellPro’ technique. We also found significantly higher numbers of CD34/45^+^ cells in the CD34^+^ enriched final fraction when using only one, 1.080, Percoll density gradient in the first separation step. Using one density separation step followed by the ‘CellPro’ technique, we obtained an average of 3×10^6^ purified CD34/45^+^ cells from samples containing 8.5×10^8^ nucleated cells. Granulomonocytic progenitors (CFU‐GM) and mixed progenitors (CFU‐GEMM) c
Who reads The purification of CD34^+^ cells from human cord blood: comparison of separation techniques and cytokine requirements for optimal growth of clonogenic progenitors?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- PIERRE CHARBORD; ISOBEL NEWTON; LAURENT VOILLAT; JEAN PATRICK SCHAAL; PATRICK HERVE
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0007-1048)
- Published
- 1996
- Language
- EN
- Field
- Medicine (Health Sciences)