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Direct Profiling of Phospholipids and Lysophospholipids in Rat Brain Sections After Ischemic Stroke by Hay-Yan J. Wang; Cheng Bin Liu; Hsuan-Wen Wu; Jr Shin Kuo is a Chemistry article available to read on EtoBox.

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## Abstract Stroke, a deleterious cerebrovascular event, is caused by a critical reduction in the blood flow to the brain parenchyma that leads to brain injury and loss of brain functions. The inflammatory responses following ischemia often aggravate the neurological damage. Several pro‐inflammatory mediators released after stroke are closely related to the metabolism of phospholipids. In this study we directly profiled the changes in phospholipids in the infarcted rat cerebral cortex 24 hours after middle cerebral artery occlusion (MCAO) using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS). Several phosphatidylcholine (PC) species and sphingomyelin (SM) were significantly decreased after infarction. The cationization pattern of the remaining PCs showed a prominent shift from a mostly potassiated or protonated form to a predominantly sodiated pattern. Stroke also elevated the lysophosphatidylcholines (LPCs) and heme in tissue. The isobaric pairs in PC and LPC classes were resolved by masses through their respective alkali metal adducts in the presence of CsCl. The major fatty acyl LPC species were also structurally confirmed by MALDI‐MS/

Who reads Direct Profiling of Phospholipids and Lysophospholipids in Rat Brain Sections After Ischemic Stroke?

Author

Hay-Yan J. Wang; Cheng Bin Liu; Hsuan-Wen Wu; Jr Shin Kuo

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0951-4198)

Published

2010

Language

EN

Field

Chemistry (Physical Sciences)