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De Novo Designed Self-Assembling Rhodamine Probe for Real-Time, Long-Term and Quantitative Live-Cell Nanoscopy by Jie Zhang; Heng Shi; Chen Huang; Le Mei; Qiang Guo; Ke Cheng; Pingzhou Wu; Dan Su; Qingxin Chen; Shenglong Gan; Cecilia Ka Wing Chan; Jiahai Shi; Jian Lin Chen; Chung Hang Jonathan Choi; Shao Q. Yao; Xian-Kai Chen; Ben Zhong Tang; Jufang He; Hongyan Sun is a Engineering article available to read on EtoBox.

What is De Novo Designed Self-Assembling Rhodamine Probe for Real-Time, Long-Term and Quantitative Live-Cell Nanoscopy about?

Super-resolution imaging provides a powerful approach to image dynamic biomolecule events at nanoscale resolution. An ingenious method involving tuning intramolecular spirocyclization in rhodamine offers an appealing strategy to design cell-permeable fluorogenic probes for super-resolution imaging. Nevertheless, precise control of rhodamine spirocyclization presents a significant challenge. Through detailed study of the structure-activity relationship, we identified that multiple key factors control rhodamime spirocyclization. The findings provide opportunities to create fluorogenic probes with tailored properties. On the basis of our findings, we constructed self-assembling rhodamine probes for no-wash live-cell confocal and superresolution imaging. The designed self-assembling probe Rho-2CF3 specifically labeled its target proteins and displayed high ring-opening ability, fast labeling kinetics ( 80 folds), which is very difficult to be realized by the existing methods. Using the probe, we achieved highcontrast super-resolution imaging of nuclei and mitochondria with a spatial resolution of up to 42 nm. The probe also showed excellent photostability and proved ideal for real-time

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Author

Jie Zhang; Heng Shi; Chen Huang; Le Mei; Qiang Guo; Ke Cheng; Pingzhou Wu; Dan Su; Qingxin Chen; Shenglong Gan; Cecilia Ka Wing Chan; Jiahai Shi; Jian Lin Chen; Chung Hang Jonathan Choi; Shao Q. Yao; Xian-Kai Chen; Ben Zhong Tang; Jufang He; Hongyan Sun

Publisher

American Chemical Society (ACS)

Published

2023

Language

EN

Field

Engineering (Physical Sciences)