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Targeting of an Apical Endosomal Protein to Endosomes in Madin–Darby Canine Kidney Cells Requires Two Sorting Motifs by K.E. Gokay; J.M. Wilson is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Targeting of an Apical Endosomal Protein to Endosomes in Madin–Darby Canine Kidney Cells Requires Two Sorting Motifs about?
The efficient sorting and targeting of endocytosed macromolecules is critical for epithelial function. However, the distribution of endosomal compartments in these cells remains controversial. In this study, we show that polarized Madin–Darby canine kidney (MDCK) cells target the apical endosomal protein endotubin into an apical early endosomal compartment that is distinct from the apical recycling endosomes. Furthermore, through a panel of site‐directed mutations we show that signals required for apical endosomal targeting of endotubin are composed of two distinct motifs on the cytoplasmic domain, a hydrophobic motif and a consensus casein kinase II site. Endotubin‐positive endosomes in MDCK cells do not label with basolaterally internalized transferrin or ricin, do not contain the small guanosine triphosphate‐binding protein rab11, and do not tubulate in response to low concentrations of brefeldin‐A (BFA). Nevertheless, high concentrations of BFA reversibly inhibits the sorting of endotubin from transferrin and cause colocalization in tubular endosomes. These results indicate that, in polarized cells, endotubin targets into a distinct subset of apical endosomes, and the targeting
Who reads Targeting of an Apical Endosomal Protein to Endosomes in Madin–Darby Canine Kidney Cells Requires Two Sorting Motifs?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- K.E. Gokay; J.M. Wilson
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 1398-9219)
- Published
- 2000
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)