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In Vivo Gene Delivery to Articular Chondrocytes Mediated by an Adeno‐associated Virus Vector by M. Ulrich‐Vinther; M. R. Duch; K. Søballe; R. J. O'Keefe; E. M. Schwarz; F. S. Pedersen is a Medicine article available to read on EtoBox.

What is In Vivo Gene Delivery to Articular Chondrocytes Mediated by an Adeno‐associated Virus Vector about?

## Abstract __Purposes__. (1) To investigate the efficiency of direct in vivo adeno‐associated virus (AAV) vector‐mediated gene transduction to chondrocytes in relation to normal and injured articular cartilage. (2) To evaluate the effects of ultra‐violet light‐activated gene transduction (LAGT) in chondrocytes in vivo. (3) To determine dissemination of active rAAV vector after intra‐articular administration. __Methods__. Rabbit knees with either normal or injured cartilage received an intra‐articular injection with 1.5 × 10^12^ infectious rAAV‐eGFP particles. The right knees received rAAV‐eGFP alone, whereas the left knees were given LAGT‐treatment. The transduction efficiencies were determined at 1 and 3 weeks after infection by fluorescence‐activated cell scanning. The occurrence of active shedding was monitored in serum and various tissues. __Results__. After 1 week, 7% of the chondrocytes in normal cartilage were transduced by direct rAAV transduction technique. Chondrocytes in cartilage defects demonstrated higher transduction rates compared to chondrocytes in normal cartilage. LAGT increased the cellular eGFP expression in the internal zones to 12%, but did not have any effe

Who reads In Vivo Gene Delivery to Articular Chondrocytes Mediated by an Adeno‐associated Virus Vector?

Author

M. Ulrich‐Vinther; M. R. Duch; K. Søballe; R. J. O'Keefe; E. M. Schwarz; F. S. Pedersen

Publisher

Elsevier Science; Wiley (John Wiley & Sons); Wiley-Blackwell; Wiley (ISSN 0736-0266)

Published

2004

Language

EN

Field

Medicine (Health Sciences)