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Can I read Emulsion PCR: Techniques and Applications on EtoBox?
Emulsion PCR: Techniques and Applications by Rashmi Kanagal-Shamanna is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Emulsion PCR: Techniques and Applications about?
Emulsion PCR (EmPCR) is a commonly employed method for template amplifi cation in multiple NGSbased sequencing platforms. The basic principle of emPCR is dilution and compartmentalization of template molecules in water droplets in a water-in-oil emulsion. Ideally, the dilution is to a degree where each droplet contains a single template molecule and functions as a micro-PCR reactor. Here, we discuss the basic principles, advantages, and challenges of applications of emPCR in clinical testing. We describe the methods of preparation and enrichment of template-positive Ion PGMTM Template OT2 200 Ion SphereTM Particles (ISPs) on the Ion Personal Genome Machine ® (PGMTM) System. For routine clinical testing, following library generation, we employ the automated Ion OneTouchTM System that includes the Ion OneTouchTM 2 and the Ion OneTouchTM ES instruments for template generation and enrichment of template-positive ISPs, respectively.
Who reads Emulsion PCR: Techniques and Applications?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Rashmi Kanagal-Shamanna
- Publisher
- Springer New York : Imprint : Humana
- Published
- 2016
- Language
- EN
- ISBN
- 9781493980307
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)